Fins Are an Applicable Organ for PCR-based Diagnosis of Koi Herpesvirus Disease in Clinical Fish

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  • Ito Takafumi
    Tamaki Laboratory, Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency
  • Hirakiuchi Hiroshi
    Coastal Fisheries Group, Shimane Prefectural Fisheries Technology Center
  • Yuasa Kei
    Diagnosis and Training Center for Fish Diseases, National Research Institute of Aquaculture, Fisheries Research Agency

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Abstract

We investigated the applicable organ to detect DNA of cyprinid herpesvirus 3 (CyHV-3), the causative agent of koi herpesvirus disease (KHVD). CyHV-3 DNA was detected at the highest positive rate in the caudal fin among several organs including gills, kidney and spleen of the fish sequentially sampled after experimental exposure to the virus. Moreover, CyHV-3 DNA was detected in the caudal and pectoral fins of all dead fish collected in a natural KHVD case. Since fins are an easily accessible organ, they can be an applicable organ for PCR-based diagnosis of KHVD in clinical fish.

Journal

  • Fish Pathology

    Fish Pathology 49 (4), 194-197, 2014

    The Japanese Society of Fish Pathology

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