Continuous Lactic Acid Production from Raw Starch in a Fermentation System Using a Reversibly Soluble-autoprecipitating Amylase and Immobilized Cells of<i>Lactobacillus casei</i>
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- HOSHINO Kazuhiro
- Department of Chemical Engineering, Faculty of Engineering, Niigata University
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- TANIGUCHI Masayuki
- Department of Chemical Engineering, Faculty of Engineering, Niigata University
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- MARUMOTO Hideji
- Department of Chemical Engineering, Faculty of Engineering, Niigata University
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- SHIMIZU Kazuyuki
- Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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- FUJII Michihiro
- Department of Chemical Engineering, Faculty of Engineering, Niigata University
書誌事項
- タイトル別名
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- Continuous lactic acid production from raw starch in a fermentation system using a reversibly soluble-autoprecipitating amylase and immobilized cells of Lactobacillus casei.
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説明
Lactic acid was continuously produced from raw starch using a combination of a reversibly soluble-autoprecipitating amylase (D-AS) depending on pH and Lactobacillus casei entrapped in κ-carrageenan. Lactic acid was produced continuously by a novel reactor system, which consisted of a turbine-blade reactor with a cylindrical stainless steel net, a mixing vessel, and a separation vessel. The gel beads with entrapped lactic acid bacteria were held on the cylindrical net equipped in the main reactor throughout cultivation. D-AS was separated continuously from a solution containing lactic acid by self-sedimentation in the separation vessel and it was returned to the main reactor for repeated use. In the continuous lactic acid production from raw starch, the lactic acid productivity was 3.1g/l/hr at a dilution rate (D)) of 0.1hr-1 and the value was about 3.1 times higher than the average of the repeated batches of lactic acid production. Although the enzymatic activity of D-AS is inactivated due to insolubilization of the enzyme by KC1 accumulated during the control of pH in the reactor, it is possible to recover the enzymatic activity by replacing a part of the old broth with a new one. This continuous production system using the novel reactor system may be widely applicable to the production of useful materials from solid substrates with other microorganisms for fermentation than lactic acid bacteria.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 55 (2), 479-485, 1991
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206462231808
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- NII論文ID
- 110006325379
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- NII書誌ID
- AA00515312
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- COI
- 1:CAS:528:DyaK3MXhsVWmtLw%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
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- OpenAIRE
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- 使用不可