直接蛍光抗体法によるαおよびβ型溶血性Streptococcus spp.の識別

  • 河原 栄二郎
    高知大学農学部水族病理学講座 北里大学水産学部水産微生物学講座
  • 楠田 理一
    高知大学農学部水族病理学講座

書誌事項

タイトル別名
  • Direct fluorescent antibody technique for differentiation between .ALPHA.- and .BETA.-hemolytic Streptococcus spp.
  • チョクセツ ケイコウ コウタイホウ ニ ヨル アルファ オヨビ ベータガタ ヨ

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抄録

Direct fluorescent antibody technique (FAT) was evaluated as a differentiation method between α-hemolytic Streptococcus sp. (KUSUDA et al., 1976; KUSUDA et al., 1978) and β-hemolytic Streptococcus sp. (MINAMI et al., 1979; KITAO et al., 1981; OHNISHI and Jo, 1981; UGAJIN, 1981; NAKATSUGAWA, 1983) in this report. The staining titer of fluorescent antibodies were examined by using heat-fixed smears of α-and β-hemolytic Streptococcus sp., S. iniae, S. equisimilis and fish pathogens other than Streptococcus. The fluorescent antibody against α-hemolytic Streptococcus sp. had a titer of 1: 10, 000 and reacted only to α-hemolytic Streptococcus sp. The fluorescent antibody against β-hemolytic Streptococcus sp. had a titer of 1: 1, 000 and reacted only to β-hemolytic Streptococcus sp. and S. iniae. The data presented here suggest that direct FAT can be utilized for differentiation of α- and β-hemolytic Streptococcus spp., and that β-hemolytic Streptococcus sp. has a common antigen with S. iniae.

収録刊行物

  • 魚病研究

    魚病研究 22 (2), 77-82, 1987

    日本魚病学会

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