書誌事項
- タイトル別名
-
- Characteristics and pathogenicity of a virus isolated from yellowtail fingerlings showing ascites.
- フクスイショウ オ テイスル ブリ チギョ カラ ブンリサレタ ウイルス ニ
この論文をさがす
抄録
In early summer, 1983, an acute disease characterized by ascites occurred among yellowtail fingerlings, Seriola quinqueradiata, cultured in a farm in the Seto Inland Sea, Japan, and accordingly virological investigations were carried out in July.<BR>All three inoculants prepared from the viscera of the fish with ascites produced cytopathic effects (CPE) of pyknosis on RTG-2, CHSE-214 and EK-1 cells within 1.5-5 days after inoculation at 20°C. The virus replicated in CHSE-214 and EK-1 cells at temperature between 5-30°C. Electron micrographs revealed pentagonal or hexagonal particles of 62-69 nm. The virus was resistant to pH 3, pH 11, ether and chloroform and was stable at 56°C for 30 minutes. Replication of the virus was not inhibited by 5-Iodo-2'-deoxyuridine. Neutralization tests with antisera proved that this virus resembled to IPNV.<BR>Pathogenicity of the virus was studied using yellowtail fingerlings (average body weight 1.1 g). Fish were exposed to the virus by immersion for 1 hour in seawater containing106.6 TCID50 /ml of the virus. The fish infected with the virus showed mortality of 62 % at water temperature of 20°C and 14 % at 25°C, while controls showed mortality of only 4 % at 20°C and 10% at 25°C, respectively. Moribund fish infected with the virus were often accompanied by severe ascites and hemorrhage in the liver. The virus reisolated from all the infected fish died.<BR>The results strongly suggest that this virus was the causative agent of the ascites of yellowtail fingerlings. The agent was tentatively named yellowtail ascites virus (YAV).
収録刊行物
-
- 魚病研究
-
魚病研究 19 (4), 231-238, 1985
日本魚病学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206462381568
-
- NII論文ID
- 10004770114
- 130000858993
-
- NII書誌ID
- AN00063165
-
- ISSN
- 18817335
- 0388788X
- http://id.crossref.org/issn/0388788X
-
- NDL書誌ID
- 3023769
-
- 本文言語コード
- ja
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可