Isolation and Characterization of a Thermostable Aminopeptidase (Aminopeptidase T) from<i>Thermus aquaticus</i>YT-1, an Extremely Thermophilic Bacterium

DOI DOI 被引用文献1件 オープンアクセス
  • MINAGAWA Etsuo
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • KAMINOGAWA Shuichi
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • MATSUZAWA Hiroshi
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • OHTA Takahisa
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • YAMAUCHI Kunio
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

書誌事項

タイトル別名
  • Isolation and characterization of a thermostable aminopeptidase(aminopeptidase T) from Thermus aquaticus YT-1, an extremely thermophilic bacterium.

この論文をさがす

説明

A thermostable aminopeptidase, called aminopeptidase T, from the extract of Thermus aquaticus YT-1 was purified and characterized. The enzyme had a dimeric structure, its relative molecular mass being 108, 000 by gel filtration, and 48, 000 by SDS-PAGE. The optimum pH of the enzyme activity was in the range of 8.5 to 9.0. The enzyme was significantly thermostable as it still retained 60% of its original activity even after heat treatment for 20 hr at 80°C. The enzyme activity was inhibited by metal-chelating agents. The enzyme had a low substrate specificity.

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

  • CRID
    1390001206462747776
  • NII論文ID
    110006323892
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.52.1755
    10.1080/00021369.1988.10868919
  • COI
    1:CAS:528:DyaL1cXlvVKjs7s%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ