Isolation and characterization of membrane-bound l-sorbose dehydrogenase form Gluconobacter melanogenus UV10

DOI DOI 被引用文献9件 オープンアクセス

書誌事項

タイトル別名
  • Isolation and characterization of membrane-bound L-sorbose dehydrogenase from Gluconobacter melanogenus UV10.
  • Isolation and characterization of membrane-bound l-sorbose dehydrogenase from Gluconobacter oxydans UV10
公開日
1991
DOI
  • 10.1271/bbb1961.55.363
  • 10.1080/00021369.1991.10870609
公開者
公益社団法人 日本農芸化学会

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説明

Membrane-bound L-sorbose dehydrogenase was purified from the membrane fraction of Gluconobacter melanogenus UV10. The reaction with 2, 6-dichlorophenolindophenol or phenazine methosulfate as acceptor was formulated as; L-sorbose + acceptor →-sorbosone + reduced acceptor.<br> The enzyme was purified by DEAE-Cellulose, DEAE-Sepharose, and Sephadex G-200 column chromatographies after differential solubilization with Triton X-100 and Tween 80, and appeared as a single band in a SDS-PAGE gel at the molecular weight of about 58, 000. It was most active at pH 7.0 and 48°C, and rather stable at around 7.0 in a buffer containing 200 mM L-sorbose, below 30°C. It showed high substrate specificity for L-sorbose, and its activity was stimulated by Fe3+ and Co2+, and inhibited by Cu2+, quinine, mono-iodoacetate, and sodium fluoroacetate.

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詳細情報 詳細情報について

  • CRID
    1390001206463009792
  • NII論文ID
    110006325364
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.55.363
    10.1080/00021369.1991.10870609
  • COI
    1:CAS:528:DyaK3MXhvVSqu78%3D
  • ISSN
    18811280
    00021369
    http://id.crossref.org/issn/00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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