A new enzymatic method of acrylamide production.

DOI DOI Web Site 被引用文献13件 オープンアクセス

書誌事項

公開日
1982
DOI
  • 10.1271/bbb1961.46.1183
  • 10.1080/00021369.1982.10865240
公開者
公益社団法人 日本農芸化学会

この論文をさがす

説明

To produce acrylamide from acrylonitrile by use of a new enzyme, nitrile hydratase, a number of nitrile-utilizing microorganisms were screened for the enzyme activity by an intact cell system. An isobutyronitrile-utilizing bacterium, strain B23, showed the best productivity among 1 86 strains tested. The strain was identified taxonomically as Pseudomonas chlororaphis. The culture and reaction conditions for the production were studied for the strain. Under the optimum conditions, 400 grams/liter of acrylamide was produced in 7.5hr. The yield was nearly 100% with a trace amount of acrylic acid. The cell-free extract of the strain showed strong activity of nitrile hydratase toward acrylonitrile and extremely low activity of amidase toward acrylamide.

収録刊行物

被引用文献 (13)*注記

もっと見る

詳細情報 詳細情報について

問題の指摘

ページトップへ