Protease-Catalyzed Synthesis of Oligo-<scp>l</scp>-Glutamic Acid from <scp>l</scp>-Glutamic Acid Diethyl Ester

DOI DOI 被引用文献2件 オープンアクセス
  • Aso Keiichi
    Department of Applied Biological Science, Science University of Tokyo
  • UEMURA Takashi
    Department of Applied Biological Science, Science University of Tokyo
  • SHIOKAWA Yutaka
    Department of Applied Biological Science, Science University of Tokyo

書誌事項

タイトル別名
  • Protease-catalyzed synthesis of oligo-L-glutamic acid from L-glutamic acid diethyl ester.

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説明

A synthesis of L-glutamic acid oligopeptide from L-glutamic acid-α, γ-diethyl ester hydrochloride is demonstrated by the use of papain and α-chymotrypsin. An examination of this polymerization reaction by turbidimetry, a micro-biuret assay and gel chromatography showed that effective synthesis was achieved at pH 7-8.5 in phosphate buffer at 25-35°C for both enzymes. It was revealed by gel chromatography that a higher molecular peptide fraction was accumulated during the reaction as a precipitate, which was determined to be peptides composed of 5 to 9 glutamic acid residues. When 100 mM of the substrate was incubated with 10 μM of papain, more than an 80% overall reaction yield (42% as a precipitate) was attained after 24 hr of the reaction.

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詳細情報 詳細情報について

  • CRID
    1390001206464638464
  • NII論文ID
    110006323477
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.52.2443
    10.1080/00021369.1988.10869064
  • COI
    1:CAS:528:DyaL1MXitVOntQ%3D%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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