Effect of protease digestion on the activity of sugar-depleted enzymes prepared with endo-β-N-acetylglucosaminidase from Flavobacterium sp.
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- YAMAMOTO Kenji
- Department of Food Science and Technology, Kyoto University
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- TAKEGAWA Kaoru
- Department of Food Science and Technology, Kyoto University
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- KUMAGAI Hidehiko
- Department of Food Science and Technology, Kyoto University
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- TOCHIKURA Tatsurokuro
- Department of Food Science and Technology, Kyoto University
書誌事項
- タイトル別名
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- Effect of protease digestion on the activity of sugar-depleted enzymes prepared with endo-.BETA.-N-acetylglucosaminidase from Flavobacterium sp.
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説明
Endo-β-N-acetylglucosaminidase, purified to homogeneity from the culture nitrate of a Flavobacterium sp., liberated the carbohydrate chains from yeast invertase. About 90% of the carbohydrate associated with this glycoprotein was removed by the endo-β-N-acetylglucosaminidase. The native and carbohydrate-depleted enzymes were compared and found to exhibit similar catalytic activities, thermal stabilities and pH-activity profiles. However, the carbohydrate-depleted invertase was more susceptible to proteases with relatively broad specificities such as subtilisin and pronase, as found on examination of the enzyme activity and electrophoresis. On the other hand, trypsin did not have such an effect on the enzyme activities of the native and carbohydrate-depleted enzymes.<br> The endo-β-N-acetylglucosaminidase also released carbohydrate chains from the purified β-N-acetylhexosaminidase of Penicillium oxalicum. Although the native and carbohydrate-depleted β-N-acetylhexosaminidases did not differ significantly in their stabilities or pH-activity profiles, the carbohydrate-depleted form was more susceptible to proteolysis by subtilisin, pronase and trypsin. From these results, it would appear that the carbohydrate of a glycosylated enzyme plays a role in protecting the enzyme from proteolysis.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 51 (6), 1481-1487, 1987
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206465633408
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- NII論文ID
- 130000025023
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- COI
- 1:CAS:528:DyaL2sXlslWnsb0%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可