Two Forms of α-Glucosidase from Rice Seeds at the Milky Stage

DOI DOI 被引用文献1件 オープンアクセス
  • YAMASAKI Yoshiki
    Institute for Agricultural and Biological Sciences, Okayama University
  • SUZUKI Yukio
    Institute for Agricultural and Biological Sciences, Okayama University

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タイトル別名
  • Two forms of .ALPHA.-glucosidase from rice seeds at the milky stage.

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説明

Two forms of α-glucosidase were isolated from rice seeds at the milky stage by a procedure that included fractionation with ammonium sulfate, DEAE-cellulose column chromatography, CM-cellulose column chromatography, SP-Sephadex column chromatography and preparative disc gel electrophoresis. The isolated a-glucosidases were designated as α-gluco-sidase I and α-glucosidase II, and were glycoproteins containing 5.5% and 3.4% carbohydrate, respectively. The molecular weight and isoelectric point were: 78900 and pH 9.3 for α-glu-cosidase I, and 47300 and pH 9.3 for α-glucosidase II. They were found to be homogeneous on polyacrylamide disc gel electrophoresis. The two enzymes hydrolyzed maltose, maltotriose, phenyl α-maltoside, amylose and soluble starch to liberate glucose, but did not act on sucrose. Their enzymes produced maltotriose and 4-α-nigerosyl-glucose from maltose as main α-glucosyltransfer products. They hydrolyzed amylose to liberate α-glucose.

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詳細情報 詳細情報について

  • CRID
    1390001206466223744
  • NII論文ID
    130003416348
  • DOI
    10.1271/bbb1961.44.707
    10.1080/00021369.1980.10864024
  • COI
    1:CAS:528:DyaL3cXktVarsL8%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
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    • Crossref
    • CiNii Articles
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    使用不可

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