Stepwise introduction of regulatory genes stimulating production of .ALPHA.-amylase into Bacillus subtilis: Construction of an .ALPHA.-amylase extrahyper producing strain.
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- HITOTSUYANAGI Kazumasa
- Shimonoseki Women's Junior College
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- YAMANE Kunio
- Institute of Applied Microbiology, The University of Tokyo
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- MARUO Bunji
- Institute of Applied Microbiology, The University of Tokyo
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説明
The production of extracellular u-amylase in Bacillus subtilis is probably regulated by many genetic elements, such as amyR, tmrA7, pap, amyB and sacU. Additional genetic elements, C-108 and A-2 for production of the α-amylase were found in D-cycloserine and ampicillin resistant mutants (C108 and A2) of B. subtilis 6160, respectively. Strain C108 in-creased the production of α-amylase about 5 times and protease about 80 times compared to parental 6160 strain. Strain A2 showed a nearly 6-fold increased α-amylase production.<br> These genetic elements displayed a synergistic effect with other genetic factors in production of extracellular α-amylase when these elements were transferred by DNA mediated trans-formation. By stepwise introduction of these and other genetic elements into B. subtilis 6160 by transformation and mutation, strains with higher a-amylase producing activity were ob-tained. The finally obtained strain, T2N26, produced about 1, 500_??_2, 000 times more α-amylase than parental 6160 strain.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 43 (11), 2343-2349, 1979
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206466329600
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- NII論文ID
- 130003416158
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- COI
- 1:CAS:528:DyaL3cXjslGrsQ%3D%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
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