Riboflavin Biosynthesis by <i>Candida robusta</i>

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  • TAKAO Shoichi
    Laboratory of Applied Microbiology, Faculty of Agriculture, Hokkaido University

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  • Riboflavin Biosynthesis by Candida robusta
  • Part III. Effect of Metal Ion and Necessity of CaCO<sub>3</sub>

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Metal ions had little influence on riboflavin production by Candida robusta, except that Ag, Cu and Hg were strongly inhibitory. The inhibiting action of iron on its production was comparatively small, so that any previous treatment for the removal of iron from the medium was not necessary. Addition of CaCO3 was found to be essential in media containing sucrose or acetate as a carbon source, as pointed out in the previous paper.1) This effect of CaCO3 was attributed to its neutralizing action. When the reaction of the medium was kept at the optimum pH, about 7.0, high yields of riboflavin could be obtained without the presence of CaCO3.

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