Studies on Metabolic Pathway of NAD in Yeast
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- TAKEI Shuzo
- Central Research Laboratories, Sankyo Co., Ltd.
書誌事項
- タイトル別名
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- Part III. Purification of Yeast 5'-Nucleotidase
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説明
Electrophoretically homogeneous preparation of yeast 5'-nucleotidase, one of NAD metabolizing system, was obtained from yeast autolysate by ammonium sulfate fractionation, chromatography, gel filtration and zone electrophoresis. The preparation had strong NAD-splitting activity, namely nucleotide pyrophosphatase activity.<br> Throughout purification steps, the ratio of the two activities were kept constant and they could not be separated even by treatments with EDTA, urea, thioglycol and alkaline buffer. These seem to suggest that both activities of 5'-nucleotidase and nucleotide pyrophosphatase localize on a single protein.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 31 (8), 917-923, 1967
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206468173696
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- NII論文ID
- 130003522800
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- COI
- 1:CAS:528:DyaF2sXltVGgu7c%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- 使用不可