Repeated-batch conversion of raw starch to ethanol using amylase immobilized on reversibly soluble-autoprecipitating carrier and flocculating yeast cells

DOI DOI 被引用文献5件 オープンアクセス
  • HOSHINO Kazuhiro
    Graduate School of Natural Science and Technology, Niigata University
  • TANIGUCHI Masayuki
    Department of Chemical Engineering, Faculty of Engineering, Niigata University
  • MARUMOTO Hideji
    Department of Chemical Engineering, Faculty of Engineering, Niigata University
  • FUJII Michihiro
    Department of Chemical Engineering, Faculty of Engineering, Niigata University

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タイトル別名
  • Repeated batch conversion of raw starch to ethanol using amylase immobilized on a reversible soluble-autoprecipitating carrier and flocculating yeast cells.

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説明

A raw-starch-digesting amylase, Dabiase K-27, was immobilized covalently on an enteric coating polymer (hydroxypropyl methylcellulose acetate succinate: AS) as a carrier which is autoprecipitating in an insoluble state below pH 4 as well as reversibly soluble-insoluble depending on pH. Dabiase immobilized on AS (D-AS) showed a sharp response of solubility to slight changes of pH without decrease in enzymatic activity. Moreover, D-AS in an insoluble state had good properties of sedimentation and a large portion of D-AS spontaneously precipitated after 10 min at pH 4.<br> D-AS was used successively for repeated ethanol production from raw starch, in which D-AS and flocculating yeast cells were separated simultaneously from a product solution by sedimentation in a reactor with a conical bottom. In the five batches of 10% raw starch, the total amount of ethanol produced from 150 g of raw starch was 61 g, a value of which corresponds to the average ethanol productivity of 0.85 g/l/hr. The repeated ethanol production by a combination of D-AS and flocculating yeast cells is a promising procedure for effectively using the enzyme and recovering the product solution economically in a heterogeneous culture system containing a solid substrate.

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詳細情報 詳細情報について

  • CRID
    1390001206468808576
  • NII論文ID
    110006324428
  • NII書誌ID
    AA00515312
  • DOI
    10.1271/bbb1961.53.1961
    10.1080/00021369.1989.10869593
  • COI
    1:CAS:528:DyaL1MXltlams7o%3D
  • ISSN
    18811280
    00021369
    http://id.crossref.org/issn/00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

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