Amperometric Titration of Starch with Iodine

DOI Open Access
  • KOBAYASHI Tsuneo
    Department of Agricultural Chemistry, Tokyo University of Agriculture and Technology
  • TAKU Akio
    Department of Agricultural Chemistry, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • Part IV Mechanism of Action of β-Amylase on Amylose An Evidence for the Multi-Chain Mechanism by Amperometric Titration with Iodine

Search this article

Description

The action pattern of β-amylase on amylose was studied by the amperometric titration with iodine. Partially hydrolyzed amylose with the degree of polymerization about 150 was hydrolyzed by crystalline sweet potato βamylase at 35°C and pH 4.8. Aliquots of the reaction mixture were taken at various time intervals during β-amylolysis, and subjected to amperometric titration. Amperograms showed that electric currents at the horizontal segments in titration curves were increased as the substrate was hydrolyzed by the enzyme. Results indicated that the β-amylase is operating by a multi-chain mechanism.

Journal

Keywords

Details 詳細情報について

  • CRID
    1390001206469068032
  • NII Article ID
    130003415212
  • DOI
    10.1271/bbb1961.29.616
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

Report a problem

Back to top