Detection of α-<scp>L</scp>-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-α-<scp>L</scp>-arabinofuranoside

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  • Detection of .ALPHA.-L-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-.ALPHA.-L-arabinofuranoside.

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For the specific detection of α-L-arabinofuranosidase (α-L-AFase)activity in isoelectric focused gels, 6-bromo-2-naphthyl-α-L-arabino-furanoside (BN-α-L-Araf) was synthesized by the condensation of 2, 3, 5-tri-0-benzoyl-α-L-arabinofuranosyl bromide and 6-bromo. 2-naphthol. α-L-AFase activity had been detected in a gel after isoelectric focusing by using the synthesized BN-α-L-Araf as a substrate, and the detection for the enzyme activity was more sensitive than protein detection with Coomassie Brilliant Blue R-250.

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