Concentration of Docosahexaenoic Acid from Fish Oils Using Geotrichum sp. FO347-2.
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- KINOSHITA Haruka
- Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University
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- OTA Yasuhide
- Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University
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抄録
Ceotrichum sp. FO347-2 could use refined sardine oil as a sole carbon source. Dry cell mass reached a maximum of 0.788 g per g of the oil added for 72 h. Total weight of the cellular lipids was largest around 24 h, when the contents of triglyceride and free fatty acid were 63.6 and 22.2%, respectively. Docosahexaenoic acid (DHA) was incorporated and concentrated in the cellular lipids, and the content reached 25.9% for 24 h, adding sardine oil containing 12.3% DHA. DHA and eicosapentaenoic acid were also concentrated in residual lipids outside the cells. Using tuna head oil containing 26.8% DHA, FO347-2 was compared with Candida guilliermondii FO726A with respect to DHA incorporation. FO347-2 and FO726A accumulated similar amounts of DHA, i.e. 53 and 55 mg, respectively, in 1 g of freeze-dried cells after 24-h cultivation at 30°C. The recovery rates of DHA from the tuna oil for FO347-2 and FO726A were 19.4 and 19.7%, respectively.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (5), 1022-1026, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206469924096
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- NII論文ID
- 110002680388
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXktFais78%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5782430
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- PubMed
- 11440112
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可