Stimulation of Pectolytic Enzyme Formation of <i>Erwinia aroideae</i> by an Active Factor in Carrot Extracts

DOI DOI Open Access
  • TOMIZAWA Haruo
    Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
  • IZAKI Kazuo
    Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
  • TAKAHASHI Hajime
    Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

Bibliographic Information

Other Title
  • Stimulation of Pectolytic Enzyme Formation of Erwinia aroideae by an Active Factor in Carrot Extracts
  • Part II. Partial Purification of the Factor and the Stimulating Effect of Some Other Compounds

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Description

Formation of pectinase system in Erwinia aroideae was stimulated to-considerable extent when the cells were incubated in a pectin medium containing carrot extracts. The active factor in the extract was purified about 30 fold by ethanol precipitation, and further purification was achieved by ninhydrin treatment, charcoal adsorption, dialysis and gel filtration with Sephadex G-10. Although crude carrot extract preparation also stimulated protease formation in this organism, no stimulating activity for protease formation was found in the purified factor. Acetate and butyrate which had been shown to stimulate pectinase formation, were found to stimulated protease formation as well. Pectinase formation by this organism was also stimulated by polyamines and inorganic phosphate to a considerable extent.

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Details 詳細情報について

  • CRID
    1390001206470666752
  • NII Article ID
    130003415307
    130003523422
  • DOI
    10.1271/bbb1961.34.1064
    10.1271/bbb1961.32.1355
  • COI
    1:CAS:528:DyaE3cXlt1yisro%3D
    1:CAS:528:DyaF1MXhslajsQ%3D%3D
  • ISSN
    18811280
    00021369
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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