Effect of Diethylpyrocarbonate on Lactobacillus casei and Its Phage Jl

DOI オープンアクセス
  • MURATA Akira
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Saga University
  • TANAKA Ryohei
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Saga University Tôyo Seisakusho Co., Ltd.
  • KANEGAWA Toshinobu
    Laboratory of Applied Microbiology, Department of Agricultural Chemistry, Saga University Kitakyushu Yakult Seizo Co., Ltd.

書誌事項

タイトル別名
  • Effect of Diethylpyrocarbonate on <i>Lactobacillus casei</i> and Its Phage Jl

この論文をさがす

説明

Diethylpyrocarbonate (DEPC) exhibited a bacteriostatic effect at concentrations between 0.5 mM and 8 mM and a bactericidal effect at concentrations above 10 mM on Lactobacillus casei S-1. At the bacteriostatic concentrations the bacteria began to grow after the added DEPC had disappeared from the medium by self-decomposition, whereas at the bactericidal concentrations such growth was not observed even after long-term incubation.<br> DEPC inactivated free phage J1 within several min; 10, 20 and 30mM of DEPC inactivated the phage by 90, 99.9 and 100%, respectively. The inactivation by DEPC was enhanced slightly by high temperature and markedly by low pH. Metal ions and chelating agents rendered negligible effects on the inactivation percentage.<br> DEPC did not cause strand scissions in the phage DNA, as exhibited by sucrose gradient centrifugation analysis. It changed the serum-blocking power of the phage, and this change was thought to be due to the structural alteration of phage tail-proteins.<br> The results are discussed in relation to the elimination of active phages from the dairy cultures.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390001206471312256
  • NII論文ID
    130003524529
  • DOI
    10.1271/bbb1961.38.367
  • COI
    1:CAS:528:DyaE2cXkt1WmurY%3D
  • ISSN
    18811280
    00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ