Comparison of protein precipitants for the determination of free amino acids in plasma.
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- OHARA Ikuo
- Life Sciences Laboratories, Central Research Laboratories, Ajinomoto Co.
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- ARIYOSHI Shujiro
- Life Sciences Laboratories, Central Research Laboratories, Ajinomoto Co.
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説明
The present study was undertaken to select the most preferred deproteinizing agent for microbiological or chromatographic determination of free amino acids in plasma. The highest quantities of amino acids were obtained from samples treated with picric acid, ethanol or sulfosalicylic acid. It was also found from examining the recoveries of 14C-labeled amino acids added to plasma following treatment with picric acid, ethanol and sulfosalicylic acid that one kind of deproteinizing agent is not equally effective with all amino acids. Picric acid treatment gave the most reliable results for determination of amino acids except the basic amino acids, alanine and tryptophan. Sulfosalicylic acid was the most recommended depro-teinizing agent for basic amino acids and ethanol was good for the assay of alanine and trypto-phan. The response of n-amino acids to the deproteinizing agents tested was not always similar to that of their L-isomers.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 43 (7), 1473-1478, 1979
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206471374464
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- NII論文ID
- 130003526566
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- COI
- 1:CAS:528:DyaE1MXlsFertb8%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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