Occurrence of Transaminations with Pyruvate and α-Ketoglutarate in Acetobacter suboxydans
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- TOCHIKURA Tatsurokuro
- Department of Agricultural Chemistry, Kyoto University Department of Food Science and Technology, Kyoto University
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- TACHIKI Takashi
- Department of Agricultural Chemistry, Kyoto University Department of Food Science and Technology, Kyoto University
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- NAKAHAMA Kazuo
- Department of Agricultural Chemistry, Kyoto University
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- BAICH Annette
- Department of Biochemistry, Oregon State University
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- CHELDELIN Vernon H.
- Department of Biochemistry, Oregon State University
書誌事項
- タイトル別名
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- Occurrence of Transaminations with Pyruvate and α-Ketoglutarate in <i>Acetobacter suboxydans</i>
- Occurrence of transamination with pyruvate and α-ketoglutarate in Acetobacter suboxidans
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説明
Transaminations between L-amino acids and pyruvate or α-ketoglutarate(α-KGA) were observed in a cell-free extract of Acetobacter suboxydans (Gluconobacter suboxydans IFO 3172). The level of the activities of transaminations with pyruvate was greatly influenced by the kinds and amounts of nitrogen sources for growth media. The enzymic activities of transaminations with pyruvate in glutamate-grown cells were extremely higher than in yeast extract-grown cells. Nutritional components decreasing the activities were presumed to be some of amino acids and ammonium ion present in yeast extract. The activities of transaminations with α-KGA were not so variable in the presence or absence of the components.<br> The optimum pH of transaminations with pyruvate was in the range of 5.0_??_5.5 and that of the reaction with α-KGA in 8.0_??_8.5. The optimum temperature of the former was 65°C and that of the latter about 45°C. Some other different properties were also recognized between the two kinds of reactions.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 37 (5), 1161-1168, 1973
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206472193024
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- NII論文ID
- 130004785893
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- COI
- 1:CAS:528:DyaE3sXltVams78%3D
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- ISSN
- 18811280
- 00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- 使用不可