Expression of the Cellulase (FI-CMCase) Gene of<i>Aspergillus aculeatus</i>in<i>Escherichia coli</i>

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  • Expression of the Cellulase (FI-CMCase) Gene of Aspergillus aculeatus in Escherichia coli.

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FI-CMCase cDNA of Aspergillus aculeatus was expressed in Escherichia coli by using the tac promoter of E. coli. Transformants of E. coli harboring a plasmid pHEM06 containing mature form FI-CMCase cDNA produced FI-CMCase in the cytoplasm of the cells. The enzyme from E. coli cells was purified to yield 56% and it was immunological identical to that of FI-CMCase purified from A. aculeatus.

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