Induction of 4-Hydroxycinnamate Decarboxylase in Klebsiella oxytoca Cells Exposed to Substrates and Non-substrate 4-Hydroxycinnamate Analogs.
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- HASHIDOKO Yasuyuki
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- TANAKA Tomoko
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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- TAHARA Satoshi
- Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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The 4-hydroxycinnamate decarboxylase (4-HCD)-inducing activity of several substrate analogs toward Klebsiella oxytoca was investigated. Four E-cinnamateclass compounds, E-4-hydroxycinnamic acid (1), caffeic acid (2), ferulic acid (3) and E-2,4-dihydroxycinnamic acid (4), all of which were accepted as substrates, all of which were accepted as substrates of 4-HCD, enable K. oxytoca cells to induce the decarboxylase at a 2.0 mM concentration, while five non-substrate compounds of the E-cinnamate class so far tested were completely in-active. However, 6-hydroxy-2-naphthoic acid (11) and 7-hydroxycoumarin 3-carboxylic acid (14), both of which are non-cinnamate-class analogs of the substrate, acted as strong 4-HCD inducers, even at a 0.5 mM concentration. The 4-HCD-inducing activities of compounds 11 and 14 at 0.5 mM were 10-12-fold higher than that of substrate 1. Compound 11 maintained its 4-HCD-inducing activity toward cultured cells through the late-log and stationary phases, unlike 1 that induced 4-HCD only in the early log phase. SDS-PAGE electrophoresis of protein mixtures from the cultured cells exposed to any 4-HCD inducer indicated that the 21.5 kDa protein was always present.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (12), 2604-2612, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206472836864
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- NII論文ID
- 110002693332
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD38XjtVyhtg%3D%3D
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- ISSN
- 13476947
- 09168451
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- HANDLE
- 2115/15847
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- NDL書誌ID
- 6027201
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- PubMed
- 11826954
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可