Mass Production of Pure Gibberellin A<sub>1</sub>by Phaeosphaeria sp. L487 and the Fungal Preparation of [U-<sup>13</sup>C]Gibberellin A<sub>1</sub>

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  • KENMOKU Hiromichi
    The United Graduate School of Agricultural Sciences, Iwate University (Yamagata University)
  • OOZONE Tadaomi
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University
  • SUGAI Tomoharu
    Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University
  • SASSA Takeshi
    The United Graduate School of Agricultural Sciences, Iwate University (Yamagata University) Department of Bioresource Engineering, Faculty of Agriculture, Yamagata University

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  • Mass Production of Pure Gibberellin A1 by Phaeosphaeria sp. L487 and the Fungal Preparation of [U-13C]Gibberellin A1.

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The mass production of pure gibberellin A1 (GA1) by shake-culturing Phaeosphaeria sp. L487 was investigated. Its GA1 production was markedly influenced by natural nitrogen sources and NH4NO3. When the fungus was cultured in an 8% glucose−1.5% oatmeal−0.1% NH4NO3−0.5% KH2PO4−0.1% MgSO4·7H2O medium for 3 weeks, the amount of GA1 in the culture filtrate was up to ca. 200 μg/ml: the addition of safflower oil to the culture medium two weeks after inoculation prolonged the GA1-production period to produce 300 μg/ml. Further preparation of [U-13C]GA1 as a tool for the analysis of a complex of GA1 and its binding protein was attempted by using the fungus. The fungal culture in a [U-13C]glucose-oatmeal medium gave 6 mg of crystalline 13C-enriched GA1. Its 13C-enrichment of ca. 75% and 1JCC values were determined by NMR spectrometry.

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