Cytokinin-induced Gene Expression in Cultured Green Cells of Nicotiana tabacum Identified by Fluorescent Differential Display.

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  • KIMURA Takuma
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • NAKANO Takeshi
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • TAKI Noriyuki
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • ISHIKAWA Mayuko
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • ASAMI Tadao
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)
  • YOSHIDA Shigeo
    Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research)

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The cell growth and plastid development of cultured green tobacco cells were maintained by the phytohormone cytokinin. After subculture into cytokinin-free medium, when cytokinin treatment was resumed, physiological changes induced by cytokinin were analyzed. Changes in chlorophyll biosynthesis and photosynthetic gene expression were observed 1 week after cytokinin induction, and changes in cell growth were observed 2 weeks after cytokinin induction. Two cytokinin-induced genes (cig) were isolated from these cells using the fluorescent differential display technique. Northern analysis confirmed that expression of these cig was induced by both natural and synthetic cytokinins. The expression of cig1 was also induced by abscisic acid, and its cDNA sequence was similar to the proline dehydrogenase gene. The expression of cig2 is specific to cytokinin and is not induced by other phytohormones. The amino acid sequence encoded by cig2 is similar to the GDP/GTP exchange factor eIF2B, which regulates translation initiation. The expression of these cig suggests a complex induction system involving cytokinin and other phytohormones.

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