IEC-6 Intestinal Cell Death Induced by Bovine Milk .ALPHA.-Lactalbumin
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- XU Meiyu
- Department of Food Science, Faculty of Applied Biological Science, Gifu University
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- SUGIURA Yoshimasa
- Department of Food Science, Faculty of Applied Biological Science, Gifu University
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- NAGAOKA Satoshi
- Department of Food Science, Faculty of Applied Biological Science, Gifu University
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- KANAMARU Yoshihiro
- Department of Food Science, Faculty of Applied Biological Science, Gifu University
Bibliographic Information
- Other Title
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- IEC-6 Intestinal Cell Death Induced by Bovine Milk α-Lactalbumin
- IEC 6 Intestinal Cell Death Induced by Bovine Milk アルファ Lactalbumin
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Abstract
Potent inhibition of cell proliferation was found for commercial preparations of bovine α-lactalbumin on cultured intestinal cell line IEC-6 albeit lot-dependent. The inhibition was irreversible and a single exposure to the culture medium containing α-lactalbumin of an active lot for a period as short as 30 min was enough to provoke cell death, possibly through apoptosis. The oligomer fraction from size exclusion chromatography was significantly robust, while the monomer fraction remained totally inert, in inducing cell death. Incubation at 37 °C for 5 d with 30% trifluoroethanol in acetate, pH 5.5, in a slowly rotating test tube rendered the monomer fraction cytotoxic. Again, the resulting inhibitory activity was found in the oligomer fraction from size exclusion chromatography, with emergence of subtle peaks at 22- and 30-kDa. Furthermore, the occurrence of SDS-stable 30-kDa as well as 20-kDa bands on electrophoresis was a common feature for α-lactalbumin with the activity inducing cell death. Thus, a certain dimeric state can be implicated in the cytotoxicity of bovine α-lactalbumin.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (6), 1082-1089, 2005
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206472987776
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- NII Article ID
- 130000030292
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD2MXmtFKmsL0%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7349465
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- PubMed
- 15973038
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed