Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisidae

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  • ZIMMER Thomas
    Department of Biotechnology, The University of Tokyo Friedrich Schiller University Jena, Institute of Physiology and Institute of Pathophysiology
  • OGURA Atsushi
    Department of Biotechnology, The University of Tokyo
  • TAKEWAKA Tsuyoshi
    Department of Biotechnology, The University of Tokyo
  • ZIMMER Rose-Marie
    Department of Biotechnology, The University of Tokyo Friedrich Schiller University Jena, Institute of Physiology and Institute of Pathophysiology
  • OHTA Akinori
    Department of Biotechnology, The University of Tokyo
  • TAKAGI Masamichi
    Department of Biotechnology, The University of Tokyo

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タイトル別名
  • Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae.
  • Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in<i>Saccharomyces cerevisiae</i>

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  In this study, we addressed the question of which genes are transcriptionally co-regulated upon proliferation of the endoplasmic reticulum, as induced by an experimental overexpression of cytochrome P450Cm2 (CYP52A4) in Saccharomyces cerevisiae. Using the mRNA differential display technique, six genes were found to be up-regulated: ASN2, MDJ1, YLR194c, YNL208w, YER175, and YGL121c. Genes coding for Dur1.2p, Dal2p, and Sps19p were down-regulated. Two strongly induced genes, which were found to accommodate the peroxisome box (YLR194c) and a 10-bp consensus sequence of genes involved in lipid metabolism (YNL208w) in their promoter regions, were further analyzed with respect to the course of induction, the necessity of the P450 membrane anchor for induction, and the effects of gene disruption on P450Cm2 overexpression. We found that both genes are not essential to overproduce P450Cm2, but their induction was dependent on P450Cm2 membrane integration.<br>

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