Lysyl-tRNA Synthetase of Bacillus stearothermophilus Molecular Cloning and Expression of the Gene.

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  • TAKITA Teisuke
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • SHIMIZU Naofumi
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • SUKATA Tokuo
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • HASHIMOTO Satoshi
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • AKITA Eriko
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • YOKOTA Takuya
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
  • ESAKI Nobuyoshi
    Institute for Chemical Research, Kyoto University
  • SODA Kenji
    Institute for Chemical Research, Kyoto University
  • INOUYE Kuniyo
    Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
  • TONOMURA Ben’ichiro
    Department of Food Science and Technology, Faculty of Agriculture, Kyoto University

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  • Lysyl-tRNA Synthetase of<i>Bacillus stearothermophilus</i>Molecular Cloning and Expression of the Gene

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Abstract

  The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids. The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.<br>

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