Cloning and Characterization of a Gene Complementing the Mutation of an Ethanol-sensitive Mutant of Sake Yeast.

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  • Cloning and Characterization of a Gene Complementing the Mutation of an Ethanol-sensitive Mutant of<i>Sake</i>Yeast

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Abstract

  Ethanol-sensitive mutants (es1 to es10) were isolated from sake yeast, Saccharomyces cerevisiae SY-32. These mutants were unable to grow at 7% ethanol at which the wild type strain SY-32 does grow. The mutants had a variety of fermentation rates and viabilities in the presence of ethanol. The gene ERG6, com- plementing the ethanol-sensitive mutation of es5, was cloned from an SY-32 gene library. ERG6 encodes S-adenosylmethionine: delta 24-sterol-C-methyltransferase (EC 2.1.1.41) in the ergosterol synthetic pathway. Mutant es5 had a reduced ability to synthesize ergosterol. An erg6 disruptant was also ethanol-sensitive. These results suggested that ERG6 plays an important role in the ethanol tolerance of S. cerevisiae.<br>

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