<i>Agrobacterium</i>-mediated Transformation of Javanica Rice cv. Rojolele
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- RACHMAWATI Diah
- Gene Research Center, Ibaraki University Faculty of Biology, Gadjah Mada University
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- HOSAKA Takehiko
- Gene Research Center, Ibaraki University
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- INOUE Eiichi
- Gene Research Center, Ibaraki University
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- ANZAI Hiroyuki
- Gene Research Center, Ibaraki University
書誌事項
- タイトル別名
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- Agrobacterium-mediated Transformation of Javanica Rice cv. Rojolele
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抄録
The Agrobacterium-mediated transformation system was extended to a famous Javanica rice variety, Rojolele, that is cultivated in Indonesia now. Efficient callus induction from immature and mature seeds of Rojolele did not succeed by any previous method for any rice cultivar. In this study, the callus from mature seeds of Rojolele exhibited a compact and nodular appearance on C medium after the carbon source and medium pH was modified. Scutellum-derived calli from mature seeds were co-cultivated with Agrobacterium tumefaciens strains EHA101 or LBA4404 that carried plasmid pAFT14, which contained the genes for β-glucuronidase (gus) and hygromycin resistance (hpt). Finally, the transformation efficiency of Rojolele variety using A. tumefaciens strain EHA101 (pAFT14) was improved to about 23%, similar to that of the Japonica rice variety Nipponbare. The seed fertility of transgenic Rojolele was more than 90%. The copy number of the transgene varied from one to three copies in the T0 transgenic lines. Both the gus and the hpt genes were inherited and expressed in the progeny.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (6), 1193-1200, 2004
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473307648
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- NII論文ID
- 10013286287
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 6990737
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- PubMed
- 15215580
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可