Expression of a Recombinant Molt-Inhibiting Hormone of the Kuruma Prawn<i>Penaeus japonicus</i>in<i>Escherichia coli</i>

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  • OHIRA Tsuyoshi
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • NISHIMURA Takayuki
    Department of Biology, Faculty of Science, Konan University
  • SONOBE Haruyuki
    Department of Biology, Faculty of Science, Konan University
  • OKUNO Atsuro
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • WATANABE Toshiki
    Ocean Research Institute, The University of Tokyo
  • NAGASAWA Hiromichi
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • KAWAZOE Ichiro
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • AIDA Katsumi
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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タイトル別名
  • Expression of a Recombinant Molt-Inhibiting Hormone of the Kuruma Prawn Penaeus japonicus in Escherichia coli.

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説明

The crustacean molt-inhibiting hormone (MIH) suppresses ecdysteroid synthesis by the Y-organ. The MIH of the kuruma prawn Penaeus japonicus has recently been isolated and its cDNA cloned. In this study, we expressed the MIH in Escherichia coli to obtain a large quantity of this hormone with biological activity. The MIH cDNA was processed and ligated into an expression plasmid. E. coli was transformed with this plasmid, and then the recombinant MIH (r-MIH) was expressed. The r-MIH was put through the refolding reaction and was purified by reverse-phase HPLC. N-terminal amino acid sequence and time-of-flight mass spectral analyses supported the idea that the r-MIH had the entire sequence. By in vitro bioassay using the Y-organ of the crayfish, the r-MIH was found to be comparable to natural MIH in inhibiting ecdysteroid synthesis.<br>

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