α-L-Rhamnosidase of Sphingomonas sp.R1 Producing an Unusual Exopolysaccharide of Sphingan
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- HASHIMOTO Wataru
- Research Institute for Food Science, Kyoto University
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- MURATA Kousaku
- Research Institute for Food Science, Kyoto University
書誌事項
- タイトル別名
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- .ALPHA.-L-Rhamnosidase of Sphingomonas sp. R1 Producing an Unusual Exopolysaccharide of Sphingan.
- アルファーL-Rhamnosidase of Sphingomonas sp
- α-<scp>L</scp>-Rhamnosidase of<i>Sphingomonas</i>sp. R1 Producing an Unusual Exopolysaccharide of Sphingan
- α-Rhamnosidase of Sphingomonas sp. R1 producing an unusual exopolysaccharide of sphingan
この論文をさがす
説明
A soil bacterium with α-L-rhamnosidase was isolated from a cumulative mixed culture containing a polysaccharide of gellan as a carbon source and identified to be Sphingomonas paucimobilis, known as a potent producer of gellan. The isolate (designated Sphingomonas sp. R1) produced an unusual exopolysaccharide of sphingan (denoted HWR1) distinct from gellan. The rhamnose in gellan was replaced with mannose in HWR1. The bacterium had a peculiar cell surface covered with many complicated plaits. α-L-Rhamnosidase purified from Sphingomonas sp. R1 grown in the presence of naringin was a monomer with a molecular mass of 110 kDa and most active at pH 8.0 and 50°C. The enzyme required divalent metal ions for the activity and released L-rhamnose from various rhamnosyl glycosides.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (6), 1068-1074, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473481728
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- NII論文ID
- 110002679213
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXksFCjurY%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL書誌ID
- 4514494
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- PubMed
- 9692187
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可