Purification, Properties, and N-Terminal Amino Acid Sequences of Guar Gum-degrading Enzyme from<i>Bacillus circulans</i>K-l
書誌事項
- タイトル別名
-
- Purification, Properties, and N-Terminal Amino Acid Sequences of Guar Gum-degrading Enzyme from Bacillus circulans K-1.
- Purification Properties and N Terminal
この論文をさがす
説明
A guar gum-degrading enzyme of the newly isolated Bacillus circulans K-1 was purified to an electrophoretically homogeneous state. The molecular weight of the purified enzyme was 62, 000 by SDS-PAGE. The purified enzyme was separated into at least six isozymes by isoelectric focusing and the pI of these isozymes were 5.4, 5.5, 5.6, 5.8, 6.0, and 6.2, respectively. The N-terminal amino acid sequences of the typical three of these proteins were all the same, Ala-Ser-Gly-Phe-Tyr-Val-Ser-Gly-Thr-Lys-Leu-Leu-Asp-Ala-Thr-Gly-Gln-Pro-Phe-Val-Met-Arg. The enzyme was most active at pH 6.9 and at 64°C. The enzyme was activated slightly by Al3+ and inhibited strongly by Sn2+ and Zn2+, N-bromosuccinimide, 2-mer-captoethanol, and ethylenediamine-tetraacetic acid.
収録刊行物
-
- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
-
Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (2), 251-255, 1997
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206473509248
-
- NII論文ID
- 110002678457
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DyaK2sXhs1Gqtrg%3D
-
- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
-
- NDL書誌ID
- 4153756
-
- PubMed
- 9058961
-
- 本文言語コード
- en
-
- 資料種別
- journal article
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
-
- 抄録ライセンスフラグ
- 使用不可