Stabilizing Effect of Tropomyosin on Actin during Storage at Low Temperature.
Bibliographic Information
- Other Title
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- Stabilizing Effect of Tropomyosin on Ac
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Description
The effects of tropomyosin (TM) on the stability of actin with and without heavy meromyosin (HMM) during storage at low temperature were studied by the DNase I inhibition assay. The stabilizing effect of TM on actin without ATP at 0°C was associated with the extent of binding of TM to the actin filaments that was dependent on the KCl concentrations: i.e., TM effectively depressed the denaturation of actin at a KCl concentration ranging from 0.1 to 0.5 M whereby TM can be bound to F-actin, Stabilization by TM could reduce the probability of the destabilization of actin due to a small amount of HMM. A combination of TM and a large amount of HMM strongly stabilized actin, even in a high salt concentration such as 0.6 M KCl These results indicate that tropomyosin played an important role in stabilizing actin in an actomyosin complex (natural actomyosin) during treatment with salt at a low temperature.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 61 (2), 233-237, 1997
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206473643648
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- NII Article ID
- 110002678454
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 4153753
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed