Properties of Mycelial Aggregate-specific Lectin of<i>Pleurotus cornucopiae</i>Produced in<i>Pichia pastoris</i>

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  • SUMISA Futoshi
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • IIJIMA Naoto
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • ANDO Akikazu
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • SHIGA Maiko
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • KONDO Kozue
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • AMANO Koh
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
  • NAGATA Yoshiho
    Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

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タイトル別名
  • Properties of Mycelial Aggregate-specific Lectin of Pleurotus cornucopiae Produced in Pichia pastoris
  • Properties of mycelial aggregate-specific lectin of Pleurotus cornucopiae produced in Phichia pastoris

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説明

cDNA of a mycelial aggregate-specific lectin of Pleurotus cornucopiae was expressed in Pichia pastoris, and the expression product was purified and characterized. The product was functional, and the hemagglutinating activity was inhibited most strongly by the addition of N-acetyl-D-galactosamine as was the native lectin. The native lectin is a glycoprotein having five glycosylation recognition signals, and the expression product showed slightly larger molecular mass than that of the native one due to further glycosylation.

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