Enzyme-Linked Immunosorbent Assay for Detection of Chitooligosaccharides.
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- KIM Soon-Young
- Department of Food Science and Technology, College of Agricultural Life Sciences, Seoul National University
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- SHON Dong-Hwa
- Food Chemistry and Biotechnology Division, Korea Food Research Institute
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- LEE Ke-Ho
- Department of Food Science and Technology, College of Agricultural Life Sciences, Seoul National University
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In order to detect chitooligosaccharides (COS), an enzyme-linked immunosorbent assay (ELISA) was developed. A chitooligosaccharide mixture (COSM) conjugated to bovine serum albumin was used to immunize rabbits to produce an anti-COS polyclonal antibody. By use of specific antibody and COSM-horseradish peroxidase conjugate, we established a competitive direct ELISA (cdELISA) the detection limit of which was about 0.1 μg/ml. In the cdELISA, the cross-reactivities of the specific antibody toward glucosamine, chitobiose, chitotriose, chitotetraose, chitopentaose, and chitohexaose were 0.27, 27, 75, 75, 144, and 100%, respectively, and those toward N-acetylchitobiose, N-acetylchitotriose, N-acetylchitotetraose, N-acetylchitopentaose, and N-acetylchitohexaose were 1.58, 0.005, 1.08, 0.05, and 0.40%, respectively.<br>
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (4), 696-701, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473693952
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- NII論文ID
- 110002679978
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXjtVelsLY%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5379934
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- PubMed
- 10830479
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
