Multiplicity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in the Gram-positive Polychlorinated Biphenyl Degrading Bacterium Rhodococcus rhodochrous K37
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- TAGUCHI Katsuhiko
- Environmental Molecular Biology Laboratory, RIKEN Institute
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- MOTOYAMA Masaki
- Environmental Molecular Biology Laboratory, RIKEN Institute
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- KUDO Toshiaki
- Environmental Molecular Biology Laboratory, RIKEN Institute Science of Biological Supramolecular Systems, Graduate School of Integrated Science, Yokohama City University
Bibliographic Information
- Other Title
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- Multiplicity of 2,3-Dihydroxybiphenyl Dioxygenase Genes in the Gram-positive Polychlorinated Biphenyl Degrading Bacterium<i>Rhodococcus rhodochrous</i>K37
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Abstract
Rhodococcus rhodochrous K37, a Gram-positive bacterium grown under alkaline conditions, was isolated for its ability to metabolize PCBs. Analysis revealed that it has eight genes encoding extradiol dioxygenase, which has 2,3-dihydroxybiphenyl 1,2-dioxygenase activity, and these genes were designated bphC1 to bphC8. According to the classification of extradiol dioxygenases [Eltis, L. D., and Bolin, J. T., J. Bacteriol., 178, 5930–5937 (1996)], BphC3 and BphC6 belong to the type II enzyme group. The other six BphCs were classified as members of the type I extradiol dioxygenase group. BphC4 and BphC8 were classified into a new subfamily of type I, family 3. Two linear plasmids, 200 kb and 270 kb in size, were found in K37, and the bphC6 and bphC8 genes were located in the 200 kb linear plasmid. Northern hybridization analysis revealed that the bphC1, bphC2, and bphC7 genes were induced in the presence of testosterone, the bphC6 gene was induced by fluorene, and the bphC8 gene was induced by biphenyl. All eight BphC products exhibited much higher substrate activity for 2,3-dihydroxybiphenyl than for catechol, 3-methylcatechol, or 4-methylcatechol.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (4), 787-795, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206473706368
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- NII Article ID
- 10013143593
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6930163
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- PubMed
- 15118304
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed