Characterization of Sericin Powder Prepared from Citric Acid-degraded Sericin Polypeptides of the Silkworm, Bombyx Mori
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- KURIOKA Akira
- Silk Science Research Institute
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- KURIOKA Fujie
- Silk Science Research Institute
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- YAMAZAKI Masayoshi
- Silk Science Research Institute
Bibliographic Information
- Other Title
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- Characterization of Sericin Powder Prepared from Citric Acid-degraded Sericin Polypeptides of the Silkworm,<i>Bombyx Mori</i>
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Abstract
Acid-degraded sericin powder (AC-SP) was prepared from aqueous solution containing citric acid-degraded sericin polypeptides of Bombyx mori. The morphological and biochemical properties of AC-SP were compared with those of alkali-degraded sericin powder (AL-SP) and hot-water degraded sericin powder (HW-SP). Based on an SEM analysis, AC-SP showed a thin film structure of 10–100 μm with good dispersity while AL-SP and HW-SP had a much larger thin film structure (<500 μm). The extract of AC-SP showed stronger trypsin inhibitor activity due to cocoon shell trypsin inhibitor (CSTI-IV) than that of HW-SP. The extract of AL-SP showed no CSTI-IV activity. It was found that AC-SP was a trypsin inhibitor complex powder and that the release of CSTI-IV from AC-SP depended on pH and ion strength. Similar powder materials were obtained when such organic acids as tartaric acid and succinic acid were used. These results suggest that the acid-degraded sericin polypeptides work as a protein matrix to which CSTI-IV may bind ionically.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (4), 774-780, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206473707648
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- NII Article ID
- 10013143545
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6930131
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- PubMed
- 15118302
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed