Purification, Characterization, and Molecular Cloning of a Chitinase from the Seeds of Benincasa hispida.
-
- SHIH Chao-Yun T.
- RCMI Program, Health Research Center, Department of Biology, Southern University
-
- KHAN Anwar A.
- Department of Biological Sciences, Louisiana State University and LSU Agricultural Center
-
- JIA Shifang
- RCMI Program, Health Research Center, Department of Biology, Southern University Current address: Institute of Microbiology, Academia Sinica
-
- WU Junlin
- Department of Biological Sciences, Louisiana State University and LSU Agricultural Center Current adress: Center for Agricultural Biotechnology, University of Maryland
-
- SHIH Ding S.
- Department of Biological Sciences, Louisiana State University and LSU Agricultural Center
Search this article
Description
A chitinase was purified from the seeds of Benincasa hispida, a medicinal plant also called white gourd, and a member of the Cucurbitaceae family. Purification was done by using a procedure consisting of only two fractionation steps: an acid denaturation step followed by ion-exchange chromatography. The sequence of the N-terminal forty amino acid residues was analyzed and the sequence indicated that the enzyme is a class III chitinase. The enzyme, which is a basic chitinase, is one of at least five chitinases detected in the seed extract of B. hispida. Like other class III chitinases, this enzyme also has lysozyme activity. A genomic clone of the gene encoding the enzyme was isolated and sequenced. The gene has the potential to encode a protein of 301 amino acid residues. The deduced amino acid sequence of the protein, as expected from the N-terminal amino acid sequence, shares high degrees of similarity with other class III chitinases.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 65 (3), 501-509, 2001
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206473819136
-
- NII Article ID
- 110002680295
-
- NII Book ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD3MXisFKqtLY%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 5736474
-
- PubMed
- 11330660
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed