A New Assay Using Surface Plasmon Resonance (SPR) to Determine Binding of the<i>Lactobacillus acidophilus</i>Group to Human Colonic Mucin

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  • UCHIDA Hideaki
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • FUJITANI Kenji
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • KAWAI Yasushi
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • KITAZAWA Haruki
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University
  • HORII Akira
    Department of Molecular Pathology, Tohoku University School of Medicine
  • SHIIBA Kenichi
    Department of Gastrointestinal and Colorectal Surgery, Tohoku University School of Medicine
  • SAITO Kazuya
    Department of Gastrointestinal and Colorectal Surgery, Tohoku University School of Medicine
  • SAITO Tadao
    Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University

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タイトル別名
  • A New Assay Using Surface Plasmon Resonance (SPR) to Determine Binding of the Lactobacillus acidophilus Group to Human Colonic Mucin
  • New Assay Using Surface Plasmon Resonance SPR to Determine Binding of the Lactobacillus acidophilus Group to Human Colonic Mucin
  • A new assay using surface plasmon resonance (SPR) to determine binding of the Lactobacillus acidophilius group to human colonic mucin

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A new binding assay to investigate the mechanism of adhesion of lactic acid bacteria to the human intestine was established by the surface plasmon resonance technique using a biosensor BIACORE1000. Cells of 26 strains of the Lactobacillus acidophilus group as analytes were eluted onto a sensor chip on which were immobilized biotinylated A-trisaccharide polymer probes having human A-type antigen [(GalNAcα1-3(Fucα1-2)Gal)-] or human colonic mucin of blood type A (HCM-A) as ligands. In the first screening, high adhesive affinity to the A-trisaccharide BP-probe was observed in L. acidophilus OLL2769, L. crispatus JCM8778, LA205 and LA206. In the second screening, which used HCM-A, only L. acidophilus OLL2769 and L. crispatus JCM8778 were selected as adhesive strains with specific binding ability to human A-antigen. The results indicated that some strains of the L. acidophilus group could recognize and bind the sugar chain of A-antigen structure on HCM.

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