Purification and Characterization of Aspartic Proteinase from Sunflower Seeds.
-
- PARK Hyekyeong
- Institute of Applied Biochemistry, University of Tsukuba
-
- YAMANAKA Nanae
- Institute of Applied Biochemistry, University of Tsukuba
-
- MIKKONEN Anita
- Research and Development Center of Kajaani, University of Oulu
-
- KUSAKABE Isao
- Institute of Applied Biochemistry, University of Tsukuba
-
- KOBAYASHI Hideyuki
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
この論文をさがす
抄録
Aspartic proteinases were purified from sunflower seed extracts by affinity chromatography on a pepstatin A-EAH Sepharose column and by Mono Q column chromatography. The final preparation contained three purified fractions. SDS-PAGE showed that one of the fractions consisted of disulfide-bonded subunits (29 and 9 kDa), and the other two fractions contained non-covalently bound subunits (29 and 9 kDa). These purified enzymes showed optimum pH for hemoglobinolytic activity at pH 3.0 and were completely inhibited by pepstatin A like other typical aspartic proteinases. Sunflower enzymes showed more restricted specificity on oxidized insulin B chain and glucagon than other aspartic proteinases. The cDNA coding for an aspartic proteinase was cloned and sequenced. The deduced amino acid sequence showed that the mature enzyme consisted of 440 amino acid residues with a molecular mass of 47,559 Da. The difference between the molecular size of purified enzymes and of the mature enzyme was due to the fact that the purified enzymes were heterodimers formed by the proteolytic processing of the mature enzyme. The derived amino acid sequence of the enzyme showed 30-78% sequence identity with that of other aspartic proteinases.<br>
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 64 (5), 931-939, 2000
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206473980416
-
- NII論文ID
- 110002680023
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD3cXjvFOqsb0%3D
-
- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
-
- NDL書誌ID
- 5404526
-
- PubMed
- 10879461
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可