Screening for Drosophila Proteins with Distinct Expression Patterns during Development by use of Monoclonal Antibodies.
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- KUMAGAI Takeshi
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- YOKOYAMA Hiroaki
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- GOTO Akira
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- HIROSE Junko
- Department of Food and Nutrition, Kyoto Women's University
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- KADOWAKI Tatsuhiko
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
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- NARITA Hiroshi
- Department of Food and Nutrition, Kyoto Women's University
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- KITAGAWA Yasuo
- Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- Screening for Drosophila Proteins with Distinct Exprssion Patterns during Development by use of Monoclonal Antibodies
- Screening for<i>Drosophila</i>Proteins with Distinct Expression Patterns during Development by use of Monoclonal Antibodies
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Abstract
Kc 167 is a cell line established from Drosophila embryonic hemocytes and has been shown to express many extracellular matrix (ECM) and other proteins important during development. We have screened monoclonal antibodies (mAbs) raised against heparin affinity purified proteins from conditioned medium of Kc 167 cells to identify novel proteins with important roles for development. One mAb recognized a protein expressed with temporary and tissue specific patterns during Drosophila embryogenesis and larval development. This approach is an alternative to screening of Expression Sequence Tag (EST) clones by in situ hybridization to initiate reverse genetics. In addition, a number of mAbs recognizing ECM proteins were also identified. These mAbs will be useful for biochemical and cell biological analyses of Drosophila ECM proteins.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (1), 24-28, 2000
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206474036480
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- NII Article ID
- 110002679742
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXhtVyitb4%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 5143405
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- PubMed
- 10705444
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed