The Characteristics and Applications of Recombinant Cholesterol Dehydrogenase.
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- KISHI Koji
- International Reagents Corp.
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- WATAZU Yoshifumi
- International Reagents Corp.
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- KATAYAMA Yoshiaki
- Department of Clinical Chemistry, National Cardiovascular Center Hospital
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- OKABE Hiroaki
- Department of Laboratory Medicine, School of Medicine, Kumamoto University
書誌事項
- タイトル別名
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- Characteristics and Applications of Recombinant Cholesterol Dehydrogenase
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抄録
Mass production of an r-CDH derived from Nocardia species was made possible by gene technology. (Horinouchi et al., Applied and Environmental Microbiology, 57, 1386-1393 (1991)). However, the characteristics of the r-CDH have not been studied in detail and have not been improved enough for industrial use. We accordingly characterized both the native-CDH and the r-CDH prepared from Streptomyces lividans. Both CDHs were monomers with molecular masses of 37 kDa. The Km of r-CDH was 2.50×10-3 M for cholesterol and 2.33×10-4 M for NAD. The activators of CDHs were TritonX-100 and cholate. TritonX-405, Ag+, and Zn2+ inhibited both enzymes. The residual activity of native CDH after heat treatment was 32% (37°C, 60 min), while the r-CDH showed a residual activity of 87% (37°C, 60 min). The r-CDH is an enzyme with high substrate specificity for cholesterol as well as native CDH and higher thermal stability than native CDH.<br> We have developed a novel serum cholesterol assay using the r-CDH, which permits the direct measurement of cholesterol by measuring NADH reaction products. We conclude that this r-CDH enzyme is useful and can be used to measure cholesterol in a clinical chemistry setting.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (7), 1352-1358, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474222592
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- NII論文ID
- 110002680098
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXlvFGrtbg%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5475818
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- PubMed
- 10945250
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可