Oxygen Sensitivity of NifA Protein of <i>Azospirillum lipoferum</i> FS as Suggested by Gene Cloning and Expression in <i>Escherichia coli</i>
書誌事項
- タイトル別名
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- Oxygen Sensitivity of NifA Protein of Azospirillum lipoferum FS as Suggested by Gene Cloning and Expression in Escherichia coli.
- Oxygen Sensitivity of NifA Protein of A
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説明
We cloned and sequenced a 2.8-kb SalI fragment of Azospirillum lipoferum FS as a homologue of the Klebsiella oxytoca nifA gene. The amino acid sequence deduced from an open reading frame of 1872 bases showed 91% identity to that of the A. brasilense NifA, and the putative central σ54 interaction domain was conserved as well as the C-terminal DNA-binding domain. The NifA function on the nifH promoter was examined in Escherichia coli using a combination of a nifA driver plasmid and a nifH-lacZ reporter plasmid, in which the transcriptional activation of the nifH promoter by the NifA was evaluated with the β-galactosidase activity. The A. lipoferum NifA activated the nifH promoter solely under microaerobic conditions, while the K oxytoca NifA activated it irrespective of the oxygen condition. These observations suggest that oxygen sensitivity is an intrinsic property of the A. lipoferum NifA.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (5), 768-771, 1997
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474225408
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- NII論文ID
- 110002678576
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK2sXjsVyju7k%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4219997
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- PubMed
- 9178550
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
