Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium,Pseudomonas fluorescens KUIN-1
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- OBATA Hitoshi
- Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center
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- ISHIGAKI Hitoshi
- Department of Biotechnology, Faculty of Engineering, Kansai University
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- KAWAHARA Hidehisa
- Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center
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- YAMADE Kazuhiro
- Department of Biotechnology, Faculty of Engineering, Kansai University
書誌事項
- タイトル別名
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- Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium, <i>Pseudomonas fluorescens</i> KUIN-1
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The psychrotrophic ice-nucleating bacterium, Pseudomonas fluorescens KUIN-1 respond to a decrease in temperature with the induction of proteins that are classified as cold shock proteins (CSPs). We found the function of a 26-kDa protein of the CSPs in the strain KUIN-1. In strain KUIN-1, a cold shock from 18 to 4°C induced the synthesis of the 26-kDa protein. By analysis with SDS-PAGE, it was then demonstrated that the 26-kDa protein was produced by the cells after treatment at 4°C. The 26-kDa protein was purified to apparent homogeneity by (NH4)2SO4 precipitation and some chromatographies (QA52, phenyl Superose, Superose 12, and Mono Q). The purified 26-kDa protein is composed of 6 subunits of 26.5-kDa with a molecular mass of approximately 159-kDa according to gel filtration and SDS-PAGE. The N-terminal sequence of the 26-kDa protein was Gln-Ala-Ala-Tyr-Tyr-Pro-Ala-His-His-His-Gln-Gln-Val-Gln-Gln-His-Trp-Gly-His-His-. Specifically, 26-kDa protein of the CSPs of strain KUIN-1 was very effective in protecting the cold-labile enzyme, lactate dehydrogenase against denaturation by freezing. The characteristics of 26-kDa protein are analogous to the cold-regulated protein of the plants.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (11), 2091-2097, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474308224
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- NII論文ID
- 110002679085
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXnvFymur4%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4667007
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- PubMed
- 9972230
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可