Catabolite Repression of the Xylanase Gene(xynA) Expression in Bacillus stearothermophilus No.236 and B. subtilis.
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- CHO Ssang-Goo
- Graduate School of Biotechnology, Korea University
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- CHOI Yong-Jin
- Graduate School of Biotechnology, Korea University
Bibliographic Information
- Other Title
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- Catabolite Repression of the Xylanase Gene (<i>xynA</i>) Expression in<i>Bacillus stearothermophilus</i>No. 236 and<i>B. subtilis</i>
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Abstract
Catabolite repression of the Bacillus stearothermophilus No. 236 xynA gene, encoding an extracellular xylanase, was investigated in this work. Expression of the xynA gene in the B. stearothermophilus strain was found to be subject to glucose catabolite repression, and the level of repression was about 50-fold when the relative amounts of xynA transcript synthesized on different carbon sources were analyzed. The experiments with the B. subtilis MW15 strains carrying plasmids containing the xynA::aprA fusion gene showed that the cloned xynA gene did not require any specific carbon source for its induction. Nevertheless, the expression of the cloned gene was repressed by the presence of glucose. From the nucleotide sequence of the cloned xynA gene, we found two potential catabolite responsive elements (cre) within its reading frame region (cre-1: nucleotides +160 to +173 and cre-2: +173 to +186). Furthermore, by using various deletion derivatives of the xynA::aprA fusion plasmid (pMGW23), we suggested that only the cre-2 element might play a role in the glucose catabolite repression. Repression level of the xynA gene expression in the recombinant B. subtilis strain was estimated to be about 3-fold by analysis of the amounts of xynA transcript.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (12), 2053-2058, 1999
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206474394624
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- NII Article ID
- 110002679419
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXlsFKmsQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 4949412
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- PubMed
- 10664837
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed