Complementary Effects of Bifidogenic Growth Stimulators and Ammonium Sulfate in Natural Rubber Serum Powder on<i>Bifidobacterium bifidum</i>

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  • ETOH Shin-ichi
    Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
  • SONOMOTO Kenji
    Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
  • ISHIZAKI Ayaaki
    Laboratory of Microbial Technology, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University

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  • Complementary Effects of Bifidogenic Growth Stimulators and Ammonium Sulfate in Natural Rubber Serum Powder on Bifidobacterium bifidum.

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  Natural rubber serum powder, rich in crude protein and carbohydrates, had a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254, which was unable to grow in a fully synthetic medium, B12 assay medium. Natural rubber serum powder was fractionated by ultrafiltration (molecular weight cutoff 1000). The active ultrafiltrate was further concentrated and desalted with an adsorptive microconcentrator, which adsorbs virtually all amino acids and peptides. Through this purification step, it was found that the adsorbed fraction obtained did not stimulate growth independently but acted complementarily with a small amount of ammonium sulfate. The adsorbed fraction was subsequently analyzed on reversed-phase high pressure liquid chromatography, and the activities of the eluates were measured on B12 assay medium with ammonium sulfate. Consequently, it was proved that several peptidic ingredients in the adsorbed fraction increased the growth of B. bifidum.<br>

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