Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Starch Column
書誌事項
- タイトル別名
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- Simple Affinity Purification Method for Raw Starch-adsorbable and -digesting Amylases with a Raw Strach Column.
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説明
A simple purification procedure for raw starch-adsorbable and -digesting amylases (RSAs) was devised. The method depended on an affinity column, which was prepared by mixing raw corn starch and Hyflo Super-Cel. RSAs were specifically adsorbed on the matrix, and eluted with a buffer containing 1% β-cyclodextrin. This column could be used to purify RSAs from Streptomyces thermo-cyaneoviolaceus and a recombinant strain of E. coli.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (10), 1717-1719, 1996
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474596736
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- NII論文ID
- 110002678353
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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