Role of the DNA Sequence Downstream of the Bacillus subtilis hut Promoter in Regulation of the hut Operon.
-
- EDA Shima
- Institute of Applied Biochemistry, University of Tsukuba National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI
-
- HOSHINO Takayuki
- Institute of Applied Biochemistry, University of Tsukuba
-
- ODA Masanao
- National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, MITI
Bibliographic Information
- Other Title
-
- Role of the DNA Sequence Downstream of the<i>Bacillus subtilis</i><i>hut</i>Promoter in Regulation of the<i>hut</i>Operon
Search this article
Abstract
To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G→A, +14C→T, and +23T→G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at the hut promoter.<br>
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 64 (3), 484-491, 2000
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206474706048
-
- NII Article ID
- 110002679940
-
- NII Book ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD3cXit1Gnsbw%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 5334271
-
- PubMed
- 10803944
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed