Construction and Characterization of Escherichia coli Disruptants Defective in the yaeM Gene.

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  • KUZUYAMA Tomohisa
    Institute of Molecular and Cellular Biosciences, University of Tokyo
  • TAKAHASHI Shunji
    Institute of Molecular and Cellular Biosciences, University of Tokyo
  • SETO Haruo
    Institute of Molecular and Cellular Biosciences, University of Tokyo

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  • Construction and Characterization of<i>Escherichia coli</i>Disruptants Defective in the<i>yaeM</i>Gene

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Abstract

  Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.<br>

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